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The Michelson Prize & Grants in Reproductive Biology is a $75M program that exists to incentivize research through prize philanthropy and grant funding to rapidly develop a permanent, single-dose, nonsurgical sterilant for male and female cats and dogs.

Listings for: "Renquist"

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Increasing the circulating half-life of GnRH-RIP conjugates to improve in vivo efficacy, Benjamin Renquist, PhD - Project completed

Project Description 

Increase stability and half life of GnRH-RIP conjugates to enhance cytotoxic destruction of pituitary gonadotrophs.

PI 
Benjamin Renquist, PhD, Department of Animal Sciences, University of Arizona, Tucson, AZ USA, bjrenquist@email.arizona.edu
Findings 
1. GnRH-toxin conjugates are internalized and effectively induce apoptosis in immortalized gonadotrope cell lines with abundant GnRH receptors. This efficacy in immortalized cells does not translate to efficacy in primary pituitary cells, or in in mice.
2. Based on results from this research, efficacy of GnRH-ribosome inactivating protein conjugates is limited by endosome sequestration and degradation of the internalized toxin.
3. The efficacy of GnRH-ribosome inactivating protein conjugates (measured in vitro in immortalized gonadotropes) can be increased by encouraging endosome disruption.
4. Further work is needed to test if these toxin-conjugates, with the addition of endosome disruptors, can be effective in mice, and eventually in dogs and cats, as a single-shot sterilant.
Project Status 
Project completed
Award 
$407,353 (3 years, start date 2012)

Enhancing the toxicity of GnRH- and bivalent-targeted RIP conjugates to induce sterility, Benjamin Renquist, PhD - Project in progress

Project Description 

Authors will continue their investigation of how to safely destroy gonadotropes, which are cells in the pituitary gland that produce hormones required for sperm production and ovulation in males and females, respectively. Complete gonadotrope destruction results in sterility. Because all gonadotropes express GnRH receptors, the Renquist lab is delivering toxins to gonadotropes by attaching toxins to GnRH. However, initial findings by this research group have shown that gonadotropes are resistant to targeted toxins. In this project, the researchers will focus on enhancing the efficacy of internalized toxins. Authors envision having an optimized cytotoxin for application in mice by the end of the study.

PI 
Benjamin Renquist, PhD, Department of Animal Sciences, University of Arizona, Tucson, AZ USA, bjrenquist@email.arizona.edu
Findings 
Project in progress
Project Status 
Project in progress
Award 
$395,110 (2 years, start date 2015)